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proprietary blocking solution superblocktm blocking buffer  (Thermo Fisher)


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    Thermo Fisher proprietary blocking solution superblocktm blocking buffer
    Proprietary Blocking Solution Superblocktm Blocking Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proprietary+blocking+buffer/superblock+t20++pbs++blocking+buffer/pm35438432-109-5-11
    Average 90 stars, based on 1 article reviews
    proprietary blocking solution superblocktm blocking buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Blocking Assay:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
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    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors
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    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors
    Article Snippet: Tissue was then washed with a universal horseradish peroxidase (HRP) reagent, and incubated with diaminobenzidine tetrahydrochloride (DAB) chromagen reagents for 5 min. Immunostained slides were counterstained with hematoxylin, dehydrated, and mounted with permanent mounting media (Vector Labs; Burlingame, CA). .. Details of reagents used are provided in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Component Vendor Reference Part Number Description Peroxidazed 1 Biocare Medical PX968 H, M, MM Buffered hydrogen peroxide, plus stabilizer Background Sniper Biocare Medical BS966 H, JJ, L, M, MM Purified casein combination of proteins in modified PBS with preservative and surfactant MACH 4 Universal HRP-Polymer Biocare Medical M4U534 G Biotin-free Detection DAB Chromagen Kit Biocare Medical IPK 5010 G80 intelliPATH FLX DAN Chromagen (IPC5008G3) intelliPATH FLX DAB Buffet (IPBF5009G20) 4 × 20 mL Target Retrieval Solution Low pH Dako North America Inc. S2369 Citric acid, pH 6.0 Antibody Diluent Pierce (Thermo Fisher) 37571 Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent Hemotoxylin Biocare Medical CATHE Counterstain Open in a separate window Abbreviations: topoI-pS10, topoisomerase I phosphorylates at serine 10; DAB, diaminobenzidine tetrahydrochloride; HRP, horseradish peroxidase. ..

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    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
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    Formulation:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors
    Article Snippet: Target Retrieval Solution Low pH , Dako North America Inc. , S2369 , Citric acid, pH 6.0. .. Antibody Diluent , Pierce (Thermo Fisher) , 37571 , Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent. .. Hemotoxylin , Biocare Medical , CATHE , Counterstain.

    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors
    Article Snippet: Tissue was then washed with a universal horseradish peroxidase (HRP) reagent, and incubated with diaminobenzidine tetrahydrochloride (DAB) chromagen reagents for 5 min. Immunostained slides were counterstained with hematoxylin, dehydrated, and mounted with permanent mounting media (Vector Labs; Burlingame, CA). .. Details of reagents used are provided in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Component Vendor Reference Part Number Description Peroxidazed 1 Biocare Medical PX968 H, M, MM Buffered hydrogen peroxide, plus stabilizer Background Sniper Biocare Medical BS966 H, JJ, L, M, MM Purified casein combination of proteins in modified PBS with preservative and surfactant MACH 4 Universal HRP-Polymer Biocare Medical M4U534 G Biotin-free Detection DAB Chromagen Kit Biocare Medical IPK 5010 G80 intelliPATH FLX DAN Chromagen (IPC5008G3) intelliPATH FLX DAB Buffet (IPBF5009G20) 4 × 20 mL Target Retrieval Solution Low pH Dako North America Inc. S2369 Citric acid, pH 6.0 Antibody Diluent Pierce (Thermo Fisher) 37571 Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent Hemotoxylin Biocare Medical CATHE Counterstain Open in a separate window Abbreviations: topoI-pS10, topoisomerase I phosphorylates at serine 10; DAB, diaminobenzidine tetrahydrochloride; HRP, horseradish peroxidase. ..

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Saline:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Incubation:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Behavioral and Cognitive Consequences of Obesity in Parents and Offspring in Female and Male Rats: Implications of Neuroinflammation and Neuromodulation.
    Article Snippet: Obesity is a rapidly growing public health concern that can create a family-wise burden.. This study was aimed to investigate behavioral, cognitive, neuroinflammatory, and neuromodulatory consequences of the diet and parental obesity.. Female and male Wistar albino rats were fed on either an obesogenic or standard diet for 12 weeks, beginning with weaning.

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Purification:

    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors
    Article Snippet: Tissue was then washed with a universal horseradish peroxidase (HRP) reagent, and incubated with diaminobenzidine tetrahydrochloride (DAB) chromagen reagents for 5 min. Immunostained slides were counterstained with hematoxylin, dehydrated, and mounted with permanent mounting media (Vector Labs; Burlingame, CA). .. Details of reagents used are provided in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Component Vendor Reference Part Number Description Peroxidazed 1 Biocare Medical PX968 H, M, MM Buffered hydrogen peroxide, plus stabilizer Background Sniper Biocare Medical BS966 H, JJ, L, M, MM Purified casein combination of proteins in modified PBS with preservative and surfactant MACH 4 Universal HRP-Polymer Biocare Medical M4U534 G Biotin-free Detection DAB Chromagen Kit Biocare Medical IPK 5010 G80 intelliPATH FLX DAN Chromagen (IPC5008G3) intelliPATH FLX DAB Buffet (IPBF5009G20) 4 × 20 mL Target Retrieval Solution Low pH Dako North America Inc. S2369 Citric acid, pH 6.0 Antibody Diluent Pierce (Thermo Fisher) 37571 Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent Hemotoxylin Biocare Medical CATHE Counterstain Open in a separate window Abbreviations: topoI-pS10, topoisomerase I phosphorylates at serine 10; DAB, diaminobenzidine tetrahydrochloride; HRP, horseradish peroxidase. ..

    Modification:

    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors
    Article Snippet: Tissue was then washed with a universal horseradish peroxidase (HRP) reagent, and incubated with diaminobenzidine tetrahydrochloride (DAB) chromagen reagents for 5 min. Immunostained slides were counterstained with hematoxylin, dehydrated, and mounted with permanent mounting media (Vector Labs; Burlingame, CA). .. Details of reagents used are provided in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Component Vendor Reference Part Number Description Peroxidazed 1 Biocare Medical PX968 H, M, MM Buffered hydrogen peroxide, plus stabilizer Background Sniper Biocare Medical BS966 H, JJ, L, M, MM Purified casein combination of proteins in modified PBS with preservative and surfactant MACH 4 Universal HRP-Polymer Biocare Medical M4U534 G Biotin-free Detection DAB Chromagen Kit Biocare Medical IPK 5010 G80 intelliPATH FLX DAN Chromagen (IPC5008G3) intelliPATH FLX DAB Buffet (IPBF5009G20) 4 × 20 mL Target Retrieval Solution Low pH Dako North America Inc. S2369 Citric acid, pH 6.0 Antibody Diluent Pierce (Thermo Fisher) 37571 Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent Hemotoxylin Biocare Medical CATHE Counterstain Open in a separate window Abbreviations: topoI-pS10, topoisomerase I phosphorylates at serine 10; DAB, diaminobenzidine tetrahydrochloride; HRP, horseradish peroxidase. ..



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    Details of Reagents Used for TopoI-pS10 IHC.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Developing a Phosphospecific IHC Assay as a Predictive Biomarker for Topoisomerase I Inhibitors

    doi: 10.1369/0022155418766503

    Figure Lengend Snippet: Details of Reagents Used for TopoI-pS10 IHC.

    Article Snippet: Antibody Diluent , Pierce (Thermo Fisher) , 37571 , Pierce Protein-free T20 (TBS) blocking buffer-proprietary formulation in TBS with surfactant and stabilizing agent.

    Techniques: Purification, Modification, Blocking Assay, Formulation